miR-155, miR-191, and miR-494 as diagnostic biomarkers for oral squamous cell carcinoma and the effects of Avastin on these biomarkers
Emami Naghmeh, Mohamadnia Abdolreza, Mirzaei Masoumeh, Bayat Mohammad, Mohammadi Farnoush, Bahrami Naghmeh,
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( Emami Naghmeh ) - Islamic Azad University Faculty of Basic Sciences Department of Biology
( Mohamadnia Abdolreza ) - Shahid Beheshti University of Medical Sciences National Research Institute of Tuberculosis and Lung Diseases Chronic Respiratory Diseases Research Center
( Mirzaei Masoumeh ) - Islamic Azad University Faculty of Basic Sciences Department of Biology
( Bayat Mohammad ) - Tehran University of Medical Sciences Craniomaxillofacial Research Center
( Mohammadi Farnoush ) - Tehran University of Medical Sciences Craniomaxillofacial Research Center
( Bahrami Naghmeh ) - Tehran University of Medical Sciences Craniomaxillofacial Research Center
Abstract
Objectives: Oral squamous cell carcinoma (OSCC) is one of the most common types of head and neck cancer. MicroRNAs, as new biomarkers, are recommended for diagnosis and treatment of different types of cancers. Bevacizumab, sold under the trade name Avastin, is a humanized whole monoclonal antibody that targets and blocks VEGF-A (vascular endothelial growth factor A; angiogenesis) and oncogenic signaling pathways.
Materials and Methods: This study comprised 50 cases suffering from OSCC and 50 healthy participants. Peripheral blood samples were collected in glass test tubes, and RNA extraction was started immediately. Expression levels of miR-155, miR-191, and miR-494 biomarkers in the peripheral blood of OSCC-affected individuals and healthy volunteers in vivo were evaluated using real-time PCR. The influence of Avastin on the expression levels of the aforementioned biomarkers in vitro and in the HN5 cell line was also investigated.
Results: Expression levels of miR-155, miR-191, and miR-494 in the peripheral blood of individuals affected by OSCC were higher than in those who were healthy. Moreover, Avastin at a concentration of 400 μM caused a decrease in the expression levels of the three biomarkers and a 1.5-fold, 3.5-fold, and 4-fold increase in apoptosis in the test samples compared to the controls in the HN5 cell line after 24, 48, and 72 hours, respectively.
Conclusion: The findings of this study demonstrate that overexpression of miR-155, miR-191, and miR-494 is associated with OSCC, and Avastin is able to regulate and downregulate the expression of those biomarkers and increase apoptosis in cancerous cells in the HN5 cell line
키워드
Oral cancer; MicroRNA; Avastin; Real-time polymerase chain reaction; Apoptosis
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